dot n. 多特 〔Dorothea 的昵称〕。 n. 1.点;圆点;句点;【音乐】附点〔音符后的一点,表示延长1/2拍〕。 2.一点点大的东西,小片,少量,小个子。 3.【数学】小数点;相乘的符号。 There are dots of soot on the window sill. 窗台上有一点烟灰。 Put a dot on [over] the i. 给字母 i 加上一点。 a dot of butter 一点儿奶油。 a mere dot of a child 小小的孩子。 in the year dot 〔口语〕老早以前。 off one's dot 〔俚语〕傻头傻脑的;发疯。 on the dot 〔口语〕按时;准时。 put dots on 〔口语〕使倦怠[烦闷]。 to a dot 〔美国〕完全,全部 (be correct to a dot 完全正确)。 to the dot of an i 一丝不苟地。 vt. (-tt-) 1.打点于;星罗棋布于,点缀;用点线表示。 2.〔俚语〕敲,打。 Dot your i's and j's. 写字时不要忘记在i和j字上打一点。 the sea dotted with ships 点缀着船只的海洋。 Trees dot the landscape. 树木点缀着景色。 Stars dot the sky. 星星缀满天空。 a dotted note 【音乐】附点音符。 dot a line across the page 横贯书页划一条虚线。 dot sb. in the eye 一拳打到某人眼上。 a dotted line 虚线。 dot and carry one 〔做加法时〕逢十进位〔儿童用语〕。 dot and go one 1. = dot and carry one. 2.n. (拄着丁字杖走的)瘸子;一瘸一拐的步行声。 3. 一瘸一拐的[地]。 dot down 暂且记下来。 dot one's i's and cross one's t's 打 i 的点画 t 的横线;一笔不苟,详述。 sign on the dotted line 〔美国〕在(信封、文件等留出供署名的)虚线上签名;〔比喻〕全盘接受。 n. 【法律】嫁妆,嫁资,妆奁。 adj. -al
It indicated that 76 . 5 percent of positive frequency of all the plants was achieved by using southern dot hybridization , 87 . 5 percent of the plants with gus positive activity was gotten by pcr southern blotting and 42 percent of the plants with gus positive activity was gotten by southern blotting 转基因卡那霉素抗性植株中, gus表达阳性率为43 ,随机抽样的方法进行杀虫肽和npt基因的pcr检测阳性率均为100 。经过分子生物学检测表明, southern斑点杂交76 . 5植株表现阳性。
The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna , which was first linearized by aocl . after two rounds of plaque isolation , the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization . the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10 重组转移载体dna与经bsu36i酶切线性化的修饰型病毒bm - bacpakdna共转染家蚕bmn细胞,两轮空斑筛选和纯化,获得重组病毒rbmbacph - egf ,经pcr扩增、 dna点杂交等方法鉴定证实重组病毒中已正确插入ph - egf融合基因。